FIG. 2.
Short-chain polyP assay of P. gingivalis 381, ppk-deficient mutant CW120, and complemented strain CW120C. P. gingivalis 381 (♦), CW120 (•), and CW120C (▴) were grown to mid-log phase in TSB medium with hemin and menadione. The cells were pelleted, resuspended, and incubated anaerobically in MOPS defined medium containing 0.1 mM Pi, 0.01% BSA, and 4 mg of glucose per ml. The samples were collected at 0, 1, 2, 3, and 4 h. Short-chain polyP was extracted and analyzed as described in the text. The results are averages of quadruple samples and their standard deviations are shown.