FIG. 5.
Nonopsonic cell entry by L. amazonensis requires activation of Cdc42 but not of Rac-1. (A) Number of intracellular amastigotes or FNLB in cells not transfected (UT) or transfected with Cdc42N17. (B) Number of intracellular amastigotes or FNLB in cells not transfected (UT) or transfected with RacN17. (C) Number of intracellular amastigotes or FNLB in cells not transfected (UT) or transfected with Cdc42L61. (D) Number of intracellular amastigotes or FNLB in cells not transfected (UT) or transfected with RacV12. Transfected CHO cells were exposed to amastigotes or FNLB for 60 min at 34°C and fixed, and the number of internalized particles was determined in triplicate (the data correspond to the means and SD of triplicate experiments).