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. 2002 Aug;70(8):4571–4580. doi: 10.1128/IAI.70.8.4571-4580.2002

FIG. 6.

FIG. 6.

Rac-1 activation is required for opsonin-mediated uptake of L. amazonensis. (A) Number of intracellular amastigotes (amast), fibronectin-coated amastigotes (FNam), or FNLB in CHO cells incubated at 4°C with amastigotes or FNLB for 30 min, followed by incubation at 34°C for 30 min. (B) Number of amastigotes or fibronectin-coated amastigotes internalized in the presence of 100 μM RGE or RGD peptides (Life Technologies). CHO cells were exposed to amastigotes for 60 min at 34°C. (C) Number of amastigotes or fibronectin-coated amastigotes internalized in cells not transfected (UT) or transfected with RacN17. Transfected CHO cells were exposed to amastigotes for 60 min at 34°C. (D) Number of amastigotes or IgG-coated amastigotes (IgGam) internalized in FcRII-B2 cells not transfected (UT) or transfected with RacN17. FcRII-B2-transfected CHO cells were exposed to amastigotes for 60 min at 34°C. After fixation, the number of internalized particles was determined in triplicate (the data correspond to the means and SD of triplicate experiments).