FIG. 7.

Nonopsonic cell entry by L. amazonensis does not trigger Rac-1 activation. The total endogenous levels of Cdc42 and Rac-1 were determined by Western blotting in lysates of CHO cells. The levels of activated (GTP-bound) forms of the same GTPases were determined according to their ability to bind GST-CRIB coupled to glutathione-Sepharose beads. (A) Total and activated Cdc42 and Rac-1 in wild-type CHO cells. (B) Total and activated Rac-1 in wild-type (WT CHO) and FcR-transfected (FcR CHO) cells. NT, no treatment; amast, uncoated amastigotes; FNam, fibronectin-coated amastigotes; IgGam, IgG-coated amastigotes; EGF, EGF treated.