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Expression of TcpA by V. cholerae strains grown in LB medium (pH 6.5 at 30°C for 16 h) as determined by immunoblotting experiments with anti-TcpA serum. Whole-cell lysates of O395 (lane 1), 10259 (lane 2), 10259R (lane 3), S7 (lane 4), V2 (lane 5), and a ΔtcpA strain, V1228 (lane 6), were used as antigens. An arrowhead indicates 20-kDa TcpA bands.
