TABLE 2.
List of primers used in this study
| Primera | Primer sequence (5′→3′) | Reference |
|---|---|---|
| VPI | ||
| aldA-(F) | TGTGTTTCTCGTCCAATGCC | This study |
| aldA-(R) | TCGTCTTCGCAAGATGTCGAACTTGC | |
| tagA-(F) | GGTGGTAAGATATTCACTC | 33 |
| tagA-(R) | GAGACATCTATAGAATACTGGCTG | |
| tcpP-(F) | ACTCTGTGAATATCATCCTGCC | This study |
| tcpH-(R) | CTGGGTAAGCCAAACATTGG | |
| tcpI-(F) | GCCGTCTCCGCATTAAGCTCTGCAC | 33 |
| tcpA-(F) | CACGATAAGAAAACCGGTCAAGAG | 33 |
| tcpA-(R) | ACCAAATGCACGCCGAATGGAGC | |
| tcpQ-(R) | GAGGACTGTTCTGCAATCTGCTCAT | This study |
| toxT-(F) | ACTGTATAGCAAAGCATATTCAGAGA | This study |
| toxT-(R) | CGCGGATCCATACAATCGAAAATAGGA | |
| tcpF-(F) | CTGTCAAACCATATCAGC | This study |
| tcpJ-(R) | TAAAGTAAAGCCCGAGC | |
| acfB-F | AATGTCAGACTTTGGCG | This study |
| acfC-R | CGAGATCGATAAGTCTTCC | |
| orfZ-(F) | CCATCATTCACGCCTGGGACTTCAT | 7 |
| orfW-(R) | TTCGTAATATGGCTGAGGATCATCTG | |
| int-(F) | GATAAAGAGATCAAAGCC | 33 |
| int-(R) | ATCTGCTTCCATGTGGG | |
| LJ-(F) | GTGAATCTTGATGAGACGCTCTG | 33 |
| LJ-(R) | GGTGAGCCAGGCTTATTTGGG | |
| RJ-(F) | TCGTTAGCGTGTTCGGTTCGCAGG | 33 |
| RJ-(R) | TGCTTTGTACCAGTCACAGATAG | |
| CTX | ||
| cep-(F) | TCGTTAGCGTGTCGGTTCGCAGG | This study |
| cep-(R) | TGCTTTGTACCAGTCACAGATAG | |
| orfU-(F) | GCTACATGTTTAGCTCACTG | This study |
| orfU-(R) | AGGTGCGTTAGTCATCAGCG | |
| ace-(F) | GGCGTATTGTATCTATTAAA | This study |
| ace(R) | GGTGTTATTTGATGGCTGCATG | |
| zot-(R) | GGCGGTACGAGTAAAACAAATCC | This study |
| zot-(F) | GCTTATGATGGACACCCTTTA | |
| ctxA-(F) | TTTAACGCTCGCAGGGC | 35 |
| ctxA-(R) | GGGCGAGAAAGGACGC | |
| ctxB-(F) | GGTTGCTTCTCATCATCGAACCAC | 7 |
| ctxB-(R) | GATACACATAATAGAATTAAGGAT | |
| toxR | ||
| toxR-(F) | ATGTTCGGATTAGGACAC | 13 |
| toxR-(R) | TACTCACACACTTTGATGGC |
a
F, forward primer; R, reverse primer.