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Flow cytometry scatter plot analysis of untreated peripheral blood leukocytes (A) and leukocytes treated with 20 (B) or 200 U of leukotoxin/ml (C). The upper gated region (R1) represents granulocytes, whereas the lower gated region (R2) includes PBMC. FSC-H (x axis), fluorescence channels for FSC of cells (proportional to size); SSC-H (y axis), fluorescence channels for orthogonal light scatter of cells (proportional to granularity).
