TABLE 1.
Flow-cytometric analysis of F. necrophorum leukotoxin-treated bovine peripheral PMNsa
| Toxin concn (U/ml) | No. of channels (maximum linear scale of >90% cells)
|
PI-excluding cells (%) | Cells showing phagocytosis + oxidative burst (fluorescence)b | DNA content (fluorescence)b | |
|---|---|---|---|---|---|
| FSC | SSC | ||||
| 0 | 1,000 ± 0 | 460 ± 23 | 98.2 ± 1.1 | 215.4 ± 15.4 | 95.8 ± 4.1 |
| 0.02 | 680 ± 23 | 420 ± 23 | 98.3 ± 1.4 | 237.7 ± 19.6 | 78.6 ± 1.4 |
| 0.2 | 680 ± 23 | 420 ± 23 | 98.0 ± 1.4 | 284.9 ± 23.0 | 74.5 ± 1.1 |
| 0.4 | 640 ± 23 | 440 ± 46 | 97.3 ± 1.0 | 292.5 ± 19.8 | 65.0 ± 2.7 |
| 2 | 640 ± 23 | 480 ± 23 | 93.0 ± 2.4 | 312.4 ± 28.3 | 54.8 ± 7.0 |
| 4 | 640 ± 23 | 480 ± 23 | 90.0 ± 1.8 | 357.0 ± 26.0 | 295.4 ± 6.8 |
| 20 | 640 ± 23 | 480 ± 23 | 89.4 ± 1.8 | 475.3 ± 38.9 | 300.3 ± 4.2 |
| 40 | 640 ± 23 | 480 ± 23 | 65.6 ± 11.1 | 442.4 ± 26.2 | 303.5 ± 3.7 |
| 200 | Scattered | Scattered | 10.3 ± 4.6 | 197.3 ± 14.7 | Clumps |
| 625 | No cells | No cells | 8.2 ± 3.1 | 201.8 ± 13.8 | Clumps |
| 1,250 | No cells | No cells | 4.6 ± 1.0 | 202.4 ± 7.3 | Clumps |
| 2,000 | No cells | No cells | 3.5 ± 1.8 | 214.0 ± 7.6 | Clumps |
a
Each value is the mean ± standard deviation (n = 4).
b
The phagocytosis and respiratory burst activity and DNA content are expressed as the geometric means of the fluorescence.