FIG. 7.

NF-κB activation in human monocytes preincubated with several inhibitors and exposed to A. phagocytophila. Human monocytes (10⁷ cells/ml in each well) were preincubated with H-7, H-89, genistein, and SB 203580 for 45 min and then exposed to A. phagocytophila (100 bacteria/cell) for 30 min. Nuclear extracts were prepared and used for EMSA. The data (donor 19 data) are representative of the data from five independent experiments (donors 15, 16, 17, 18, and 19) that gave similar results. The nonspecific competitor AP-2 and the specific competitor NF-κB were included in this assay to verify the effects of these competitors on binding of the NF-κB complex.