TABLE 2.
Inhibition of IL-1β, TNF-α, and IL-6 mRNAs in human PBLs, monocytes, and neutrophils in response to A. phagocytophila
| Cytokin mRNA | Inhibitor | % Inhibitiona
|
||
|---|---|---|---|---|
| PBLs | Monocytes | Neutrophils | ||
| IL-1β | SN-50 | 50 ± 12 | 8, 19 | NDb |
| MG-132 | 44 ± 7 | 23, 61 | ND | |
| SB 203580 | 60 ± 34 | 26, 32 | 0, 0 | |
| PD 098059 | 17 ± 10 | 9, 11 | ND | |
| H-7 | 95 ± 4 | 84 ± 8 | 75 ± 20 | |
| H-89 | 41, 64 | 73 ± 2 | 69 ± 11 | |
| Genistein | 49, 50 | 30, 52 | 90 ± 7 | |
| MDC | 19, 29 | 35, 54 | 64 ± 3 | |
| Verapamil | 0, 0 | 17, 27 | 12, 23 | |
| W-7 | 21, 33 | 5, 3 | 8, 16 | |
| Neomycin | 0, 0 | 16, 24 | 68 ± 12 | |
| TNF-αc | SN-50 | 74 ± 34 | 25, 53 | |
| MG-132 | 91 ± 25 | 32, 46 | ||
| SB 203580 | 93 ± 36 | 70 ± 2 | ||
| PD 098059 | 55 ± 16 | 25, 44 | ||
| H-7 | 94 ± 4 | 82 ± 5 | ||
| H-89 | 44, 72 | 68 ± 9 | ||
| Genistein | 79 ± 6 | 75 ± 13 | ||
| MDC | 32, 43 | 49, 57 | ||
| Verapamil | 67 ± 9 | 81 ± 7 | ||
| W-7 | 46, 60 | 47, 66 | ||
| Neomycin | 63 ± 7 | 0, 0 | ||
| IL-6c | SN-50 | 59 ± 24 | 42, 78 | |
| MG-132 | 95 ± 1 | 34, 82 | ||
| SB 203580 | 97 ± 1 | 94 ± 2 | ||
| PD 098059 | 44, 54 | 37, 54 | ||
| H-7 | 94 ± 3 | 90 ± 7 | ||
| H-89 | 93 ± 3 | 75 ± 3 | ||
| Genistein | 91 ± 1 | 39, 67 | ||
| MDC | 33, 34 | 11, 23 | ||
| Verapamil | 39, 42 | 18, 19 | ||
| W-7 | 38, 40 | 0, 0 | ||
| Neomycin | 37, 39 | 16, 17 | ||
a
Percentages of inhibition were calculated with the following formula: [1 − (band density in the presence of inhibitor/band density of G3PDH) ÷ (band density without inhibitor/band density of G3PDH)] × 100. The values are means ± standard deviations from three independent experiments or actual values from two independent experiments. Values that consistently were >50% are in boldface type.
b
ND, not determined.
c
Neutrophils were not induced in response to A. phagocytophila.