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. 2025 Dec 21;9:80. doi: 10.1038/s42003-025-09346-4

Fig. 5. CD36 induces the activation of Hippo-YAP signaling.

Fig. 5

A Volcano plot exhibits different lymph node metastasis-associated molecules in rank correlation with CD36 expression in TCGA database. (|Rank correlation (Pearson)| > 0.1; p < 10E-5). B Immunohistochemistry staining of YAP in 77 BC specimens collected from Sun Yat-sen University Cancer Center. Three representative cases are shown. 50× magnification and 200×magnification; Scale bar: 100 μm and 50 μm. Correlation analysis of YAP expression with CD36 expression in BC specimens is shown in the upper left corner (Spearman). n = 77, R = 0.331, p = 0.003. C, D IHC staining (A) and quantification (B) of the expression of YAP from Xenograft Models tumors. Tumors formed by CD36-infected 231, vector-transfected 231 cells. Bars correspond to mean ± standard deviation (SD). E WB analysis of the expression of YAP in indicated CD36-infected, CD36-knockdown, and vector control cell lines treated with vehicle or palmitic acid (800 μm). F Relative mRNA level of YAP1 in indicated BC cell lines treated with vehicle or palmitic acid (800 μm) for 24 h. Bars correspond to mean ± standard deviation (SD), n = 3, n.s.: not significant. G WB analysis of total YAP and phosphorylated YAP S127 expression in the indicated CD36-infected, CD36-knockdown, and vector control cells treated with vehicle (Veh) or palmitic acid (PA) for 1 h. H, I Immunofluorescence staining of nuclear YAP expression in the indicated cells treated with palmitic acid (PA, 800 μm) for 24 h. BCs cells were stained with CD36 (red), YAP (green), and DAPI (blue). Scale bar: 50 μm. Bars represent the mean ± SD of three independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant.