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. 2025 Dec 21;9:80. doi: 10.1038/s42003-025-09346-4

Fig. 8. CD36 activates YAP pathway by inducing bile acid synthesis.

Fig. 8

A GSEA of the genes related to cholesterol storage and bile acids metabolism according to dichotomized CD36 expression (Median) in the TCGA database. NES: 1.8623657 & 1.7081771; Nominal p-value < 0.0001 & 0.006 respectively. B The concentration of total bile acid (TBA) and cholesterol of indicated BC xenografts lysis. C, D WB analysis of the expression of PPARα, HMGCR, and CYP7A1 from EGFP/CD36-infected MDA-MB-231 (C) and shNC/shCD36-infected T-47D (D) cells treated with vehicle (Veh) or palmitic acid (PA, 800 μm). (E) WB analysis of the expression of PPARα, HMGCR, and CYP7A1 from Xenograft Models tumors. F WB analysis of the expression of PPARα, HMGCR, and CYP7A1 in indicated MDA-MB-231 cells treated or no treated with PPARα inhibitor (GW6471, 25 µM). G Immunofluorescence staining of nuclear YAP expression in the indicated cells treated with palmitic acid (PA, 800 μm) for 24 h. BC cells were stained with CD36 (red), YAP (green), and DAPI (blue). Scale bar: 50 μm. *p < 0.05, **p < 0.01, n.s. not significant.