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. 2025 Dec 11;17:696. doi: 10.1038/s41467-025-67304-4

Fig. 5. ZDHHC20L and ZNF354A respond to redox homeostasis and lipid peroxidation.

Fig. 5

a–e Western blot (WB) analysis of ZDHHC20 (Short and 20L isoforms) and GAPDH (loading control) in U2OS cells treated as follows: increasing concentrations of H₂O₂ (8 h) (a), increasing concentrations of RSL3 (b), siControl, siGPX4 or siZNF354A (72 h) (c), H₂O₂ (50 μM) with increasing concentrations of ferrostatin-1 (Fer-1) (d), and HeLa cells treated with proaerolysin (10 ng/ml, 1 h, washed, incubated additional 8 h) (e). Graphs in (b, d, e) show percentages of 20 L isoform and (b) ATF2 levels. Mean ± SEM; each dot represents a biologically independent experiment. Statistical analysis by two-way ANOVA, Tukey’s test; p values as indicated. Source data and entire blots are provided as a Source Data file and supplementary information.