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. 2025 Dec 12;17:720. doi: 10.1038/s41467-025-67476-z

Fig. 1. RNAPII inhibitors ectopically induce RNAPII degradation and NSN-to-SN transition in mouse oocytes.

Fig. 1

a Workflow for live sorting of NSN and SN oocytes and 3D segmentation analyses of oocyte nuclei. b Manual scoring of chromatin configurations in sorted NSN and SN oocytes after fixation and staining with Hoechst. c Automated quantification of chromatin volume in NSN and SN oocytes. p < 0.0001. d Schematic representation of oocyte nucleus (scheme: chromocenters in green, nuclear bodies in magenta, and chromatin in blue). e Automated quantification of chromatin volume in NSN and SN oocytes treated with different small molecule inhibitors or overexpressing different nuclear components. P value for NSN treated with RNase A, ActD, BRM/BRG1i and SN treated with 1,6-hexanediol, CTCF OE, DZNep, HP1β OE is less than 0.0001; P(NSN-GSK-J4) = 0.001; P(NSN-CHX) = 0.0002; P(NSN-Quarfloxin) = 0.292; P(NSN-Bleomycin) = 0.5768; P(NSN-Hinokiflvone) = 0.9574; P(NSN-TSA) = 0.0888; P(NSN-Etoposide) = 0.0294; P(SN-TET mix OE) = 0.0791; P(SN-MATR3 OE) = 0.0558; P(SN-hnRNPU OE) = 0.1106; P(SN-A-485) = 0.4147; P(SN-Hinokiflavone) = 0.0077; P(SN-DNMT mix OE) = 0.0096. f, g Immunofluorescence images of NSN oocytes treated with triptolide or α-amanitin. h–k Automated quantification of total intensity of EU, chromatin volume, total intensity of pS2, and RPB1 in NSN oocytes treated with triptolide or α-amanitin. For total intensity of EU, pS2 RPB1, and chromatin volume between NSN oocytes treated with water and triptolide or α-amanitin, p < 0.0001. *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. N.S., not significant. All box plots show median (horizontal black line), mean (small black squares), 25th and 75th percentiles (boxes), 5th and 95th percentiles (whiskers), and 1st and 99th percentiles (crosses). Statistical significance is based on unpaired, two-tailed Student’s t test (c, e, h–k). The number of oocytes analyzed is specified in italics. Scale bars are 5 µm. Source data are provided as a Source Data file.