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. 2025 Dec 12;17:720. doi: 10.1038/s41467-025-67476-z

Fig. 2. Induced SN-like nuclei recapitulate epigenetic features, chromatin interactions and developmental potential in SN oocytes.

Fig. 2

a Immunofluorescence images of NSN oocytes treated with α-amanitin. b Automated quantification of total intensity of different nuclear components in NSN oocytes treated with α-amanitin. Statistical significance is based on unpaired, two-tailed Student’s t test. P value for γH2A.X, 5hmC and KMe2 #2 is less than 0.0001; P(H4ac) = 0.0006; P(5mC) = 0.0071; P(HP1β) = 0.0133. c Chromatin contact maps of NSN, α-amanitin-treated NSN and SN oocytes. d Contact probability curves of NSN, α-amanitin-treated NSN and SN oocytes. e Manual scoring of developmental outcomes of NSN, α-amanitin-treated NSN and SN oocytes after meiotic maturation and IVF. (two-tailed Fisher’s exact test for 2-cell embryos from NSN and α-amanitin-treated NSN oocytes, p = 0.0106) *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. All box plots show median (horizontal black line), mean (small black squares), 25th and 75th percentiles (boxes), 5th and 95th percentiles (whiskers), and 1st and 99th percentiles (crosses). The number of oocytes analyzed is specified in italics. Scale bars are 5 µm. Source data are provided as a Source Data file.