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. 2025 Dec 12;17:720. doi: 10.1038/s41467-025-67476-z

Fig. 3. Targeted degradation of RNAPII is sufficient to induce NSN-to-SN transition in mouse and human oocytes.

Fig. 3

a Schematic representation of the miniTrim-Away approach. b Immunofluorescence images of NSN oocytes expressing 42B3-t21R to deplete phosphorylated RNAPII for 3 or 16 h. c–f Automated quantification of total intensity of pS2, chromatin volume, total intensity of EU and RPB1 in NSN oocytes expressing 42B3-t21R to deplete phosphorylated RNAPII for 3 or 16 h. For the total intensity of EU, pS2, RPB1, and chromatin volume between the control group and NSN oocytes expressing 42B3-t21R for 3 h or 16 h, all p < 0.0001. g Immunofluorescence images of human oocyte expressing 42B3-t21R to deplete phosphorylated RNAPII for 16 h. h Automated quantification of chromatin surface area-to-volume ratio (SA/V) in human oocytes with partially compacted and fully compacted chromatin. p = 0.0003. i Still images from time-lapse movies of human oocytes with partially compacted chromatin expressing 42B3-t21R. Time is given as hours:minutes from the start of imaging immediately after injection. Yellow dotted circles highlight the nuclei. j Automated quantification of chromatin SA/V in human oocytes with partially compacted chromatin expressing 42B3-t21R over time. Data are presented as mean values +/- SD. ***p < 0.001. ****p < 0.0001. All box plots show median (horizontal black line), mean (small black squares), 25th and 75th percentiles (boxes), 5th and 95th percentiles (whiskers), and 1st and 99th percentiles (crosses). Statistical significance is based on unpaired, two-tailed Student’s t test (c–f, h). The number of oocytes analyzed is specified in italics. Scale bars are 5 µm. Source data are provided as a Source Data file.