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. 2025 Dec 12;10:18. doi: 10.1038/s41538-025-00657-8

Fig. 7. TCA inhibits lipogenesis in 3T3-L1 cells.

Fig. 7

A CCK8 cell viability assay. B TG content. C Cell morphology observation (100×). D Oil Red O Staining (100×). E Lipid accumulation was quantified by measuring the absorbance at 510 nm. F Immunofluorescence double staining of FASN and CPT1 (100×). G, H Fluorescence intensity statistics of FASN and CPT1. I, J Relative protein expressions of FASN, CPT1, DGAT2, ATGL, P-ATGL, HSL, P-HSL. K Relative genes expressions of Atgl, Hsl, Acs, Cpt1, Srebp1c, Cebpa, Fasn, dgat2. L 3D interactions of TCA and CPT1 protein. Superscript letters (a, b, c and d) indicate significant differences between groups at P < 0.05.