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. 2025 Dec 11;17:751. doi: 10.1038/s41467-025-67438-5

Fig. 2. Proximal Vκ recombination activity is largely determined by RSS orientation in the CTCF-Nm mutants.

Fig. 2

a Schematic of the targeted inversion of the 377-kb proximal Igκ region in the CTCF-Nm Igκ+/- line. b HTGTS-V(D)J-Seq analysis of Vκ utilization frequency in CTCF-Nm Igκ+/- (top) and CTCF-Nm Igκ377kb-inv (bottom) cells. Data represent mean ± SD from three independent replicates. c,d Comparison of frequency of Vκ usage in the 377-kb proximal Igκ region in the CTCF-Nm Igκ+/- and CTCF-Nm Igκ377-inv cells, analyzed for transition from deletional to inversional joins (c) or from inversional to deletional joins (d). Data represents mean ± SD from three independent replicates. P values were calculated using unpaired, two-tailed Student’s t test. e Schematic of the targeted precise inversion of Vκ6-17 in CTCF-Nm Igκ+/- line. f Utilization frequency of Vκ6-17 in germline and Vκ6-17-inverted cells. Data represents mean ± SD from three independent replicates.