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. 2002 Aug;70(8):4692–4696. doi: 10.1128/IAI.70.8.4692-4696.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — Antiapoptotic activity of O. tsutsugamushi on THP-1 cells undergoing apoptosis caused by chemical inducers. Both live O. tsutsugamushi (LOT) and heat-killed O. tsutsugamushi (HOT) show antiapoptotic activity against beauvericin- or staurosporine-induced apoptosis. (A) Flow cytometric analysis of apoptosis after staining of THP-1 cells with 7-AAD. Live cells were not stained with 7-AAD (red), apoptotic cells were stained weakly (green), and dead cells were stained brightly (blue). (B) Analysis of the duration of the antiapoptotic activity of O. tsutsugamushi for 48 h. Apoptosis of THP-1 cells was induced with beauvericin after they had been conditioned by mock treatment (closed circles) or treatment with LPS (open circles), live O. tsutsugamushi (closed rectangles), or heat-killed O. tsutsugamushi (open rectangles) for 18 h. FSC, forward scatter.