FIG. 6.

Time course of translocation of PKC β I to early endosomes. Cells were infected with the wild type and harvested at intervals after infection. Early endosomes were isolated, and proteins were prepared for SDS-polyacrylamide gel electrophoresis and Western blotting as described in Materials and Methods. The proteins were also blotted with antibody to the transferrin receptor (upper bands). Infections were carried out for 1 min (lane 1), 3 min (lane 2), 4 min (lane 3), and 5 min (lane 4).