FIG. 3.

Effect of inoculation of NTHi 2019 and isogenic mutants on cytosolic IP levels in immortalized 16HBE14o⁻ human airway epithelial cells. Wild-type strain 2019, in comparison with the poorly invasive strain with mutations in pgmB and licD results in the increased cytosolic IP levels by means of PAF receptor activation; mutations affecting the addition of ChoP onto the oligosaccharides of the strain 2019 LOS reduce this effect (43). The 16HBE14o⁻ cells were labeled with ³H-labeled myoinositol and inoculated with the strain indicated, and the IP levels in the cytosolic fractions were assessed.