TABLE 2.
Summary of S. sonnei 53G ORFs
| ORF | Gene name | Location in sequence | % G+C | Amino acid no. | Selected homolog (accession no.) | % Identity (amino acids)a | Proposed function of 53G protein |
|---|---|---|---|---|---|---|---|
| 1 | insB | 519-16 | 54.4 | 167 | IS1 (InsB), E. coli (AJ223474) | 98 (167) | IS1 transposase |
| 2 | insA | 713-438 | 52.5 | 91 | IS1 (InsA), E. coli (AJ223475) | 100 (91) | IS1 protein |
| 3 | wzz | 788-1,720 | 36.4 | 310 | Wzz, Actinobacillus actinomycetemcomitans (AB041266) | 35 (328) | Chain length determinant |
| Wzz, E. coli (AF011911) | 26 (292) | ||||||
| 4 | wbgT | 1,756-3,069 | 36.1 | 437 | WbpO, P. aeruginosa (AF035937) | 74 (418) | UDP-GalNAc dehydrogenase |
| WcdA, Salmonella typhi (D14156) | 63 (418) | ||||||
| 5 | wbgU | 3,150-4,187 | 34.1 | 345 | WbpP, P. aeruginosa (AF035937) | 67 (343) | UDP-GlcNAc C4 epimerase |
| WcdB, S. typhi (D14156) | 65 (338) | ||||||
| 6 | wzx | 4,276-5,556 | 28.1 | 426 | Cps19CJ, Streptococcus pneumoniae (AF105116) | 21 (394) | Repeat unit transporter |
| Wzx, E. coli (AF104912) | 19 (393) | ||||||
| 7 | wzy | 5,625-6,797 | 29.8 | 390 | Cap14H, S. pneumoniae (X85787) | 25 (201) | Polysaccharide polymerase |
| 8 | IS630 | 6,894-7,925 | 52.8 | 343 | IS630 (ORF343), S. sonnei (P16943) | 99 (343) | IS630 transposase |
| 9 | wbgV | 7,958-9,202 | 29.9 | 414 | None | None | UDP-GalNAcA C5 epimeraseb |
| 10 | wbgW | 9,186-10,181 | 26.6 | 331 | WaaV, E. coli (AF019746) | 27 (237) | Glycosyl transferase |
| LgtA, N. gonorrhoeae (U14554) | 30 (142) | ||||||
| 11 | wbgX | 10,178-11,332 | 37.6 | 384 | WlbF, Bordetella bronchiseptica (AJ007747) | 55 (392) | Amino-sugar synthetase |
| Per, E. coli (AF061251) | 34 (383) | ||||||
| RfbE, V. cholerae (X59554) | 31 (380) | ||||||
| 12 | wbgY | 11,349-11,939 | 35.4 | 196 | WlbG, Bordetella pertussis (X90711) | 53 (194) | Glycosyl transferase |
| WcaJ, E. coli K-12 (U38473) | 34 (197) | ||||||
| WbaP, E. coli K30 (AF104912) | 31 (212) | ||||||
| 13 | wbgZ | 11,954-13,873 | 44.3 | 639 | WbcP, Yersinia enterocolitica (Z47767) | 68 (633) | UDP-GlcNAc C6 dehydratase C4 reductase |
| WbpM, P. aeruginosa (U50396) | 49 (657) | ||||||
| WlbL, B. pertussis (X90711) | 49 (592) | ||||||
| FlaA1, H. pylori (AE000595) | 28 (297) | ||||||
| 14 | aqpZ | 13,992-14,504 | 55.5 | 170 | ORF10P, P. shigelloides (AB025970) | 99 (146) | Water channel protein |
| AqpZ, E. coli (AE000189) | 71 (146) | ||||||
| 15 | orfA | 14,657-14,983 | 53.8 | 108 | IS629 (ORFA), S. sonnei (P16941) | 99 (108) | IS629 transposase |
| 16 | InsB | 16,706-15,486 | 55.0 | 406 | IS91 (TnpA), E. coli (X17114) | 94 (406) | IS91 transposase |
| 17 | InsA | 17,071-16,706 | 53.0 | 121 | IS91 (ORF121), E. coli (S23781) | 95 (121) | IS91 protein |
| 18 | InsB | 17,130-17,978 | 54.8 | 282 | IS911 (InsB), S. dysenteriae (AAF28127) | 99 (271) | IS911 transposase |
a
Percent identity and length of comparable sequence in the homologous protein obtained from BLAST analysis.
b
Proposed function based on the predicted presence of an enzyme that converts UDP-GalNAcA to UDP-AltNAcA (see Discussion).