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Temporal lsp operon expression levels in GAS corresponding to various divalent cation concentrations in the growth medium. The bacteria were grown as batch cultures in CDM supplemented with 10, 20, or 50 μM Zn2+ [Zn(II)]. The lsp operon expression was measured by firefly luciferase reporter activities of aliquots drawn hourly according to a protocol described in Materials and Methods and is shown as the data of one representative measurement.
