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Nonreducing sodium dodecyl sulfate-PAGE gel of CYE of H. capsulatum strain G217B developed with L-DOPA. Synthesis of a black pigment consistent with melanin occurs in situ on the gel. Lane A, commercial laccase (40 U equivalent); lane B, as for lane A but treated with KCN; lane C, 200 μg of CYE of H. capsulatum isolate G217B; lane D, as for lane C but treated with KCN. Similar results were obtained using CYE of H. capsulatum strain CIB 1980.
