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[Preprint]. 2025 Dec 3:2025.12.01.691642. [Version 1] doi: 10.64898/2025.12.01.691642

Figure 2. Characterization of the 5xFAD model.

Figure 2.

Comparisons of MRI measurements across young wild-type (WT; n = 8, 5F/3M), young 5xFAD (n = 10, 5F/5M), older WT (n = 12, 9F/3M), and older 5xFAD (n = 16, 7F/9M) mice, including brain volume (a), cerebral blood flow (CBF; b), global cerebrovascular reactivity (CVR; c), regional mean CBF maps (d), and isocortical and hippocampal CVR (e). Red and blue dots denote female and male mice, respectively. (f) Representative immunofluorescent images of α-SMA and Collagen IV staining. Scale bars: 1 mm (whole-slice views) and 50 μm (regional views). (g) Comparisons of α-smooth muscle actin (α-SMA) coverage index across young WT (n = 5, 3F/2M), young 5xFAD (n = 5, 3F/2M), older WT (n = 5, 3F/2M), and older 5xFAD (n = 5, 1F/4M) mice, which is defined as the ratio of the co-localized α-SMA and Collagen IV-positive area (α-SMA+Col-IV+) to the total Collagen IV-positive area (Col-IV+). For group-wise comparisons: *p < 0.05; **p < 0.01; n.s., not significant.