TABLE 2.
Effects of lactoferrin fragments on the adherence of S. mutans MT8148 cells to S-HA beads with or without dithiothreitol
| Lactoferrin fragment (amino acid position)b | No. of cells bound to S-HA (106)a
|
|
|---|---|---|
| Without DTT | With DDT (0.1 M) | |
| Control | 5.26 ± 0.36 | 5.18 ± 0.27 |
| Lactoferrin (1-689) | 1.01 ± 0.01 (80.8)c | 1.15 ± 0.09 (78.1)c |
| LfC (345-689) | 1.20 ± 0.15 (77.2)c | NDf |
| Lf4 (345-571) | 1.41 ± 0.21 (73.2)c | ND |
| Lf41 (345-538) | 1.78 ± 0.20 (66.2)c | ND |
| Lf42 (345-505) | 2.98 ± 0.18 (43.3)d | ND |
| Lf43 (345-472) | 5.04 ± 0.45 (4.2) | 4.66 ± 0.18 (11.4) |
| Lf44 (345-439) | 5.16 ± 0.39 (1.9) | 5.50 ± 0.48 (0) |
| Lf45 (366-571) | 5.03 ± 0.27 (4.4) | 3.51 ± 0.15 (33.3)d |
| Lf46 (399-571) | 4.34 ± 0.19 (17.5) | 2.57 ± 0.07 (51.1)c |
| Lf47 (432-571) | 5.61 ± 0.03 (0) | 3.46 ± 0.01 (34.2)d |
| Lf411 (473-538) | 4.41 ± 0.40 (16.2) | 3.81 ± 0.32 (27.6)e |
| DHFR | 4.89 ± 0.35 (7.0) | 4.78 ± 0.38 (9.1) |
a
The values were obtained at 0.5 nmol of lactoferrin fragment/ml, and are expressed as the means ± standard deviations of triplicate assays. The values in the parentheses are the percent inhibition, calculated as described in Table 1.
b
Amino acid numbering is according to Goodman and Schanbacher (6).
c
P < 0.001 compared with the control.
d
P < 0.01 compared with the control.
e
P < 0.05 compared with the control.
f
ND, not determined.