TABLE 3.

Oligonucleotide sequences used for the mutations of each cysteine residue in the Lf411 fragment

Lf411 mutant	Sequencea
Lf411-C481S (Cys481 to Ser)	5′-AACCAGACAGGCTCCTCCGCATTTGATGAATTC-3′
Lf411-C491S (Cys491 to Ser)	5′-TTCTTTAGTCAGAGCTCTGCCCCTGGGGCTGAC-3′
Lf411-C502S (Cys502 to Ser)	5′-CCGAAATCCAGACTCTCTGCCTTGTGTGCTGGC-3′
Lf411-C505S (Cys505 to Ser)	5′-AGACTCTGTGCCTTGTCTGCTGGCGATGACCAG-3′
Lf411-C515S (Cys515 to Ser)	5′-CAGGGCCTGGACAAGTCTGTGCCCAACTCTAAG-3′
Lf411-C532S (Cys532 to Ser)	5′-ACCGGGGCTTTCAGGTCCCTGGCTGAGGACGTT-3′

^aModified codons are underlined.