TABLE 1.
Negative-ion ES-MS data and proposed compositions of OdA LOS from H. somnus strains 2336, 738, and 738-lob2A1::Km grown on blood agar or in CTT
| Strain (growth medium) | Observed ions (m/z)
|
Molecular Mass (Da)
|
Relative intensity | Proposed compositiona | ||
|---|---|---|---|---|---|---|
| (M − 2H)2− | (M − H)3− | Observed | Calculatedb | |||
| 1,172.8 | 2,347.6 | 2,347.1 | 18.2 | 2Hex, 2Hep, 2EtnP, 2Kdo, lipid A | ||
| 2336 (Blood agar) | 1,253.9 | 835.4 | 2,509.0 | 2,509.3 | 15.4 | 3Hex, 2Hep, 2EtnP, 2Kdo, lipid A |
| 1,355.9 | 903.1 | 2,713.8 | 2,712.4 | 5.2 | HexNAc, 3Hex, 2Hep, 2EtnP, 2Kdo, lipid A | |
| 1,436.7 | 957.4 | 2,875.3 | 2,874.6 | 30.6 | HexNAc, 4Hex, 2Hep, 2EtnP, 2Kdo, lipid A | |
| 1,582.0 | 1,054.6 | 3,166.4 | 3,165.6 | 15.2 | NeuAc, HexNAc, 4Hex, 2Hep, 2EtnP, 2Kdo, lipid A | |
| 2336 (CTT) | 1,172.8 | 2,347.6 | 2,347.1 | 70.9 | 2Hex, 2Hep, 2EtnP, 2Kdo, lipid A | |
| 1,253.6 | 2,509.5 | 2,509.3 | 7.1 | 3Hex, 2Hep, 2EtnP, 2Kdo, lipid A | ||
| 1,355.8 | 903.5 | 2,713.5 | 2,712.4 | 7.1 | HexNAc, 3Hex, 2Hep, 2EtnP, 2Kdo, lipid A | |
| 1,436.4 | 957.2 | 2,874.7 | 2,874.6 | 14.2 | HexNAc, 4Hex, 2Hep, 2EtnP, 2Kdo, lipid A | |
| 1,581.7 | 3,165.4 | 3,165.6 | 0.2 | NeuAc, HexNAc, 4Hex, 2Hep, 2EtnP, 2Kdo, lipid A | ||
| 738 (blood agar) | 1,111.2 | 2,224.5 | 2,224.1 | 10.1 | 2Hex, 2Hep, EtnP, 2Kdo, lipid A | |
| 1,192.3 | 2,387.3 | 2,386.2 | 4.7 | 3Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,193.6 | 2,389.4 | 2,389.1 | 11.5 | ChoP, 2Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 837.6 | 2,516.0 | 2,515.1 | 4.7 | NeuAc, 2Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,293.6 | 2,589.9 | 2,589.4 | 10.1 | HexNAc, 3Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,294.6 | 2,591.1 | 2,592.3 | 6.8 | ChoP, HexNAc, 2Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 891.5 | 2,677.4 | 2,677.2 | 4.7 | NeuAc, 3Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,376.8 | 2,752.1 | 2,751.5 | 6.8 | HexNAc, 4Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 959.5 | 2,881.4 | 2,880.4 | 12.2 | NeuAc, HexNAc, 3Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,457.9 | 2,917.9 | 2,916.6 | 13.5 | ChoP, HexNAc, 4Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1014.1 | 3,045.5 | 3,042.5 | 8.1 | NeuAc, HexNAc, 4Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1068.4 | 3,208.4 | 3,207.6 | 6.8 | NeuAc, ChoP, HexNAc, 4Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 738-lob2A1::Km (blood agar) | 1,030.3 | 2,062.5 | 2,061.9 | 7.2 | Hex, 2Hep, EtnP, 2Kdo, lipid A | |
| 1,111.4 | 2,224.9 | 2,224.1 | 25.1 | 2Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,192.7 | 2,387.4 | 2,386.2 | 9.7 | 3Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,193.7 | 2,389.6 | 2,389.1 | 23.4 | ChoP, 2Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,273.3 | 2,548.3 | 2,548.4 | 4.6 | 4Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,274.8 | 2,551.9 | 2,551.3 | 9.8 | ChoP, 3Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
| 1,337.9 | 891.6 | 2,678.0 | 2,677.2 | 16.2 | NeuAc, 3Hex, 2Hep, EtnP, 2Kdo, lipid A | |
| 946.6 | 2,843.0 | 2,842.3 | 4.0 | NeuAc, ChoP, 3Hex, 2Hep, EtnP, 2Kdo, lipid A | ||
a
Abbreviations: Hex, hexose; Hep, heptose; HexNAc, N-acetylhexosamine; Kdo, 3-deoxy-d-manno-2-octulosonic acid; EtnP, phosphatidylethanolamine; ChoP, phosphorylcholine; NeuAc, N-acetylneuraminic acid.
b
Average mass units were used for calculation of molecular weight based on proposed composition as follows: Hex, 162.15; Hep, 192.17; HexNAc, 203.19; Kdo, 220.18; EtnP, 123.05; ChoP, 165.05; NeuAc, 291.00