TABLE 2.

Incorporation of [¹⁴C]NeuAc into LOS or cells by Nonidet P-40 extracts or by growth in CTT supplemented with CMP-[¹⁴C]NeuAc

Extract or growth medium	Radioactivity (dpm) of:
	LOS			Cells
	None	738-lob2A1::Km	2336	None	738-lob2A1::Km	2336	1P
None extract	27	NDb	39
N. gonorrhoeae	573	4,788	7,177
H. somnus 738	51	38	29
H. somnus 738-lob2A1::Km	24	ND	23
H. somnus 2336-R	27	ND	26
CTT-CMP-[14C]NeuAc				22	5,259	5,916	19

^aNonidet P-40 cell extracts were prepared as described for measurement of N. gonorrhoeae sialyltransferase activity (31).

^bND, not determined.