FIG. 5.

Inhibition of PMN adhesion to endothelium by GXM is stronger when endothelium is stimulated with TNF-α for 6 h than when it is stimulated for 24 h. HUVEC monolayers were stimulated with TNF-α for either 6 or 24 h and subsequently washed, and then either untreated or GXM-treated PMN were added and left for 15 min. To evaluate the relevance of both E-selectin- and CD18-mediated binding at either time point, the adhesion of PMN to endothelium was performed both after incubation of PMN with MAb IB4 (anti-CD18) and after incubation of stimulated HUVEC with MAb BBA2 (anti-E-selectin). Adhesion is expressed as the percentage of adhesion of untreated PMN (positive control) to 6- or 24-h-stimulated endothelium. The results are the averages from four experiments. ∗, P < 0.05 (comparing adhesion to the positive control of either 6- or 24-h-stimulated HUVEC set at 100%). The error bars represent standard deviations.