TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli K-12 CC118 | F− Δ(ara-leu)7697 Δ(lacZ)74 Δ(phoA)20 araD139 galE galK thi rpsE rpoB arfE(Am) recA1 | 34 |
| Y. pseudotuberculosis | ||
| YPIII | pIB1; wild type | 7 |
| YP1 | pIB1 inv::phoA(60)a Kanr | 40 |
| YP28 | pIB1 yadA::TnphoA(27)b Kanr | This study |
| YP29 | pIB1 yadA::Cm Cmlr | Dorothy Fallows |
| YP30 | pIB1 inv::Kan Kanr | Dorothy Fallows |
| YP31 | pIB1−inv::Tn10 Tetr | Dorothy Fallows |
| YP32 | pIB1 yadA::Cml inv::Kan Kanr Cmlr | Dorothy Fallows |
| Plasmids | ||
| pACYC184 | cloning vector; p15a Cmr Tetr | 12 |
| pAM226 | pACYC184 inv+ Cmr | 36 |
| pAY04 | pACYC184 yadA+ Cmr | 57 |
| pBAD18 | Cloning vector; pBAD Ampr | 19 |
| pGP704 | R6K-based suicide vector; Ampr | 39 |
| pPD259 | pAY04 yadA::TnphoA(27)a Cmr Kanr | This study |
| pPD260 | pGP704 yadA::TnphoA(27)a Ampr Kanr | This study |
| pPD284 | pBAD18 yadA+ Apr | This study |
| pRI203 | pBR325 inv+ Apr | 28 |
| pYV260 | pIB1 yadA::TnphoA(27)a Kanr | This study |
a
inv-phoA fusion expressed from the inv promoter; the number in parentheses indicates the codon of inv fused to phoA.
b
yadA-phoA fusion expressed from the yadA promoter; the number in parentheses indicates the codon of yadApst6 fused to TnphoA.