FIG. 1.
Time-dependent reduction in mean cell volume of C. albicans cells induced by exogenous addition of Hst 5. C. albicans DS1 cells (2 × 106 to 5 × 106 cells) were treated with Hst 5 in 10 mM sodium phosphate buffer (pH 7.4) at room temperature and incubated for different times. The mean cell volume (average for 20,000 cells) was measured with a Coulter Counter Multisizer II-Channelyzer at different times following addition of Hst 5 and compared with the initial value. The data was analyzed by using AccuComp software, and the changes in mean cell volume are expressed as relative cell volumes compared to the pretreatment values. The data are means ± standard deviations for three independent observations.