FIG. 2.

Hst 5-induced cell volume change is inhibited by pretreatment with DIDS. C. albicans DS1 cells (5 × 10⁶ cells) were preincubated with 1 mM DIDS for 10 min before addition of 62 μM Hst 5 in 10 mM phosphate buffer at room temperature (○). Cells were also treated only with Hst 5 (•). Control cells were preincubated with 1 mM DIDS alone (▵). The mean cell volume for each treatment and each time point was measured with a Coulter Counter Multisizer II-Channelyzer, and cell volume changes are expressed as relative cell volumes compared to pretreatment values (zero time). All of experiments were carried out at room temperature. The data are means ± standard deviations for three independent experiments.