FIG. 3.

Induction of Hst 5 expression in C. albicans DB9 cells causes reduction in mean cell size. C. albicans DB9 and DB13 cells were grown for 23 h in sucrose to induce expression of Hst 5 (DB9) and a nontoxic control protein, statherin (DB13). Cells were collected, washed, and placed into isotonic NaCl buffer, and the cell volume for each population was measured with a Coulter Counter Multisizer II-Channelyzer. Cell size is expressed as the cell diameter. The overlay profile shows the reduction in mean cell size of DB9 cells expressing Hst 5 (left arrow) compared with the mean cell size of DB13 cells expressing statherin (right arrow).