FIG. 4.

Induction of Hst 5 expression in C. albicans DB9 cells results in an increased percentage of G₁ cells. DB9 cells were assayed at different times following inoculation into cultures containing glucose (without Hst 5) (•) or sucrose (inducing Hst 5) (▪). Cells were stained with Sytox Green, and the DNA content for the total DB9 cell population under inducing (▪) and noninducing (•) conditions was measured and analyzed with the FACScan by using Modifit software (Verity Software, Inc.) to determine the percentage of G₁ cells. The viability of DB9 cells expressing Hst 5 was measured by a dilution plate assay at each time point (▵).