FIG. 6.

Hst 5 expression results in reduced Cln1 and Cln2 transcript levels. Analysis of the RT-PCR products in total RNA samples obtained from C. albicans strain DB9 (Hst 5-expressing) (A) and DB13 (statherin-expressing) (B) cells was done to examine the levels of specific G₁ cyclin transcripts. Lanes 1, 3, 5, and 7, RNA samples obtained from cultures grown on YNB-glucose (noninducing conditions); lanes 2, 4, 6, and 8, RNA samples obtained from cultures grown on YNB-sucrose (inducing conditions). The designations of the C. albicans genes examined are indicated at the top. Lane M contained molecular mass markers.