TABLE 1.

Loss of cell viability and ATP efflux from C. albicans cells pretreated with inhibitors

Treatment	ATP efflux (pmol)a		Loss of cell viability (%)b
	Without Hst 5	With Hst 5	Without Hst 5	With Hst 5
Buffer	14 ± 6	1,168 ± 513	0	98 ± 3
DIDS	46 ± 3	88 ± 20c	0	8 ± 7c
NPPB	43 ± 15	227 ± 43c	0	50 ± 6c
Niflumic acid	20 ± 17	214 ± 76c	0	49 ± 5c

^aC. albicans cells were preincubated with DIDS (1 mM), NPPB (1 mM), niflumic acid (1 mM), or buffer alone for 2 h at 37°C, and then Hst 5 (31 μM) was added to cells for 30 min. The ATP released from 10⁶ cells was quantified by using a luciferin-luciferase assay. The values are means ± standard deviations for at least three independent experiments.

^bC. albicans cells were preincubated with DIDS, NPPB, or niflumic acid (all at a concentration of 1 mM) for 10 min at 37°C and then treated with 31 μM Hst 5 for 1.5 h. Control cells were maintained in sodium phosphate buffer alone. Cells were plated after treatment to assess the loss of viability, determined as follows: [1 − (CFU of treated cells/CFU of control cells)] × 100. The values are means ± standard deviations for at least three independent experiments.

^cThe difference between cells treated with Hst 5 alone and cells pretreated with inhibitor was significant at a P value of ≤0.01, as determined by a Student t test.