FIG. 3.

B. fragilis LPS signals in the absence of TLR4. The MIP-2 secretion levels (in picograms per milliliter) of cellular supernatant following the stimulation of the cell line 10ScNCr/23 with E. coli 026:B6 LPS (100 ng/ml), S. aureus LTA (1.5 μg/ml), and B. fragilis LPS (100 ng/ml) for 4 h are shown. Detoxified LPS derived from S. enterica serovar Enteritidis (S. entero; 100 ng/ml) and LPS from E. coli 0111:B4 were included as negative controls. All experiments were repeated a minimum of three times and assayed in triplicate.