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Chronic exposure to E. coli 0111:B4 LPS does not induce alternative receptors in the absence of functional TLR4. (A) MIP-2 secretion by the 10ScNCr/23 cell line (in picograms per milliliter) following 6 weeks of continuous LPS exposure. LPS was added at 100 ng/ml, LTA was added at 1.5 μg/ml, and a no-addition negative control was included as well. (B) TNF-α readout for the identical experimental conditions. All experiments were repeated twice and assayed in triplicate.
