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. Author manuscript; available in PMC: 2006 Jun 1.
Published in final edited form as: Arthritis Rheum. 2005 Jun;52(6):1907–1919. doi: 10.1002/art.21033

Figure 5.

Figure 5

Significant decreases in joint levels of CCR1 and CCR5 messenger RNA (mRNA) and in tyrosine phosphorylation of CCR1 (pCCR1) and CCR5 (pCCR5) in Met-RANTES–treated rats with adjuvant-induced arthritis (AIA) compared with the control rats with AIA. Expression of A, CCR1 mRNA and B, CCR5 mRNA in the ankles of Met-RANTES–treated and control rats over the course of AIA. Values were normalized against mRNA for GAPDH. On days 14 and 18 after adjuvant injection, ankle homogenates from the 2 groups were immunoprecipitated with C, anti-CCR1 antibody and E, anti-CCR5 antibody and then Western blotted with antiphosphotyrosine antibody. Levels of *pCCR1 and *pCCR5 were normalized against the total CCR1 and CCR5 values, respectively. Quantification of D, *pCCR1/CCR1 and F, *pCCR5/CCR5 was obtained by the Western blot analyses performed in C and E, respectively. The intensity of the Western blot bands was quantified using Un-Scan-It software (version 5.1; Silk Scientific, Orem, UT). Values in A, B, D, and F are the mean and SEM of 5 animals per group; in C and E, each lane represents a different animal.