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LPS-induced ERK phosphorylation is increased in DAP12-deficient macrophages. Bone marrow-derived macrophages from wild-type or DAP12-deficient (DAP12 KO) mice were stimulated with 0.5 ng/ml LPS for the indicated time (min), at which time cells were lysed. Cytoplasmic extracts were analyzed using antibodies specific for p38 MAPK, p42/44 ERK and JNK, or for phosphorylated versions of these proteins. Data are representative of four independent experiments.
