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Far-Western dot immunoblot of purified Haps binding to ECM proteins. Samples were loaded with 50 μg of purified protein as follows: lane 1, BSA; lane 2, fibronectin; lane 3, fibronectin 30-kDa heparin-binding fragment; lane 4, fibronectin 45-kDa gelatin-binding fragment; lane 5, laminin; lane 6, collagen IV. Immunoblotting was performed with antiserum RabK2 after incubation of the membrane with purified Haps diluted to 10 μg/ml.
