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Binding of human complement regulators factor H and FHL-1 by C. albicans. Fungal cells (109) were incubated in NHS-EDTA and washed, and bound proteins were eluted with 0.1 M glycine. The wash (W) (lane 1) and elution (E) (lane 2) fractions were separated by SDS-PAGE and analyzed by Western blotting using a goat anti-factor H antiserum recognizing both factor H and FHL-1. A control in which factor H and FHL-1 were detected in human serum with the same antiserum is shown in lane 3.
