FIG. 3.

Determination by FACS of complement regulators surface bound to C. albicans. (A) Cells were incubated in NHS-EDTA, washed, and incubated with an antiserum recognizing both factor H and FHL-1 (anti-SCRs 1 to 4) or with an antiserum specific for SCRs 19 and 20 of factor H (anti-SCRs 19 and 20). Cells incubated in buffer instead of EDTA-NHS were used as a control. (B) Dose-dependent deposition of factor H and FHL-1 to intact C. albicans. Cells were incubated in buffer containing 5, 40, or 80% NHS-EDTA. After washing, cells were incubated with an antiserum recognizing both factor H and FHL-1 (anti-SCRs 1 to 4). Cells incubated in buffer were used as a control.