FIG. 5.

Heparin interferes with binding of factor H to C. albicans. C. albicans cells (10⁹) were incubated with ¹²⁵I-labeled factor H (approximately 20,000 cpm) in the absence and presence of increasing amount of heparin (10 to 1,000 IU/ml). Bound protein was separated from free radiolabeled protein by centrifugation through a sucrose solution, and the radioactivity of the samples was determined. Results are expressed as a percentage of cell-bound versus free protein. As a control radiolabeled protein was incubated in the absence of heparin or cells.