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Localization of the binding region in factor H and FHL-1. C. albicans cells (109) were incubated with recombinant FHL-1 (lanes 5 and 6) and the indicated deletion mutants of FHL-1 and factor H. Both wash (W) and elution (E) fractions were separated by SDS-PAGE and analyzed by Western blotting using goat anti-factor H antiserum detecting factor H, FHL-1, and deletions mutants.
