Table 1.
Lung leukocyte populations in SP-D-/- and SP-D+/+ mice at time zero, 24 and 48 hrs post-intranasal pneumococcal challenge. Leukocyte subpopulations (neutrophils, T cells, macrophages and B cells) numerated in the vicinity of inflamed bronchioles were expressed as cells per mm2 lung tissue. Leukocyte subpopulations expressed as the percentage of total lung leukocytes are shown in parenthesis. Fold increases in leukocytes subpopulations compared to time zero levels. N = 4 mice per each time point analysed for all samples. "a" denotes P < 0.01, "b" denotes P < 0.05 as compared to time zero values. "c" denotes P < 0.01, "d" denotes P < 0.05, SP-D-/- mice compared to SP-D+/+ mice at equivalent time-point.
| A) Neutrophils | SP-D+/+ mice | SP-D-/- mice | ||
| Time | Cells/mm2 | Fold increase | Cells/mm2 | Fold increase |
| Zero: | 11 +/- 2 | 9 +/-2 | ||
| 24: | 60 +/-5 a (70%) | 5.4 | 70+/-8a (53%) | 7.7 |
| 48: | 72 +/-7 a (73%) | 6.5 | 47 +/-4 b, d (59%) | 5.2 |
| B) T cells | SP-D+/+ mice | SP-D-/- mice | ||
| Time | Cells/mm2 | Fold increase | Cells/mm2 | Fold increase |
| Zero: | 9 +/-1 | 8 +/-2 | ||
| 24: | 9 +/-1 (11%) | no change | 48 +/-8 a, c (36%) | 6 |
| 48: | 8 +/-1 (8%) | no change | 16 +/-4 b, d (20%) | 2 |
| C) Macrophage | SP-D+/+ mice | SP-D-/- mice | ||
| Time | Cells/mm2 | Fold increase | Cells/mm2 | Fold increase |
| Zero: | 7 +/-1 | no change | 7 +/-1 | |
| 24: | 8 +/-2 (9%) | no change | 8 +/-3 (6%) | no change |
| 48: | 9 +/-2 (9%) | no change | 8 +/-2 (10%) | no change |
| D) B cells | SP-D+/+ mice | SP-D-/- mice | ||
| Time | Cells/mm2 | Fold increase | Cells/mm2 | Fold increase |
| Zero: | 8 +/-1 | 6 +/-1 | ||
| 24: | 8 +/-2 (9%) | no change | 7 +/-3 (5%) | no change |
| 48: | 9 +/-2 (9%) | no change | 9 +/-1 (11%) | no change |