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. 2025 Dec 19;16:2952. doi: 10.1038/s41598-025-32771-8

Table 5.

Analytical sensitivity of influenza A RT-qPCR between the laboratory-based CFX opus Real-Time PCR system and the portable MIC PCR system. Sample with Cq ≤ 45 is considered positive of AIV.

CFX Opus MIC
AIV RNA dilution Mean Cq No. of positive Mean Cq No. of positive
10− 1 21.9 3/3 20.11 3/3
10− 2 25.43 3/3 23.77 3/3
10− 3 28.9 3/3 27.29 3/3
10− 4 32.73 3/3 31.21 3/3
10− 5 36.99 3/3 35.13 3/3
10− 6 0 0/3 0 0/3
10− 7 0 0/3 39.28 1/3
10− 8 0 0/3 0 0/3