FIG. 5.

NPC1-influenced B. abortus infection. (A and B) Internalization of B. abortus. Macrophages from wild-type (black bars) or NPC1-deficient (white bars) mice were infected with virulent Ba600 (wild type) (A) or Ba604 (ΔvirB4) (B) for the periods of time indicated. Data are the average of triplicate samples from three identical experiments, and the error bars represent the standard deviation. (C) Intracellular replication of B. abortus. Macrophages from wild-type or NPC1-deficient mice were infected with Ba600 (wild type). Data points and error bars represent the mean CFU of triplicate samples from a typical experiment (performed at least four times) and their standard deviation, respectively. (D) Proliferation in mice. Wild-type (black bar) or NPC1-deficient (white bars) mice were infected with virulent B. abortus. Recovery of viable bacteria from the spleen and the weights of spleens of infected mice at 10 days postinfection are shown. Error bars indicate standard deviations.