TABLE 2.
cAMP response generated by EF after entry into the cell cytosol via the pinosomesa
| Time of incubation (min) | Radioactivity associated with the CHO cells after 125I-EF was delivered to the cells (mean cpm ± SD):
|
cAMP accumulation (mean pmol/mg of CHO cell protein ± SD):
|
||
|---|---|---|---|---|
| In the presence of calcium | In the absence of calcium | In the presence of calcium | In the absence of calcium | |
| 5 | 66,452 ± 1,145 | 62,960 ± 1436 | 1,874 ± 78 | 289 ± 11 |
| 30 | 36,348 ± 987 | 35,481 ± 932 | 1,030 ± 45 | 104 ± 7 |
| 60 | 5,624 ± 249 | 5,243 ± 193 | 137 ± 10 | 69 ± 4 |
a
For details of the experiments, see Materials and Methods.